Toxicidade animal por formaldeídeo e complucad® em ratos wistar: exposição aguda / Animal toxicity caused by formaldehyde and complucad® in wistar rats: acute exposure

Objetivos: Verificar a toxicidade em ratos Wistar expostos ao formaldeídeo a 10% e Complucad®, analisando-se as enzimas hepáticas alaninaaminotransferase e aspartatoaminotransferase, os tecidos pulmonar, renal e hepático, após período de exposição aguda. Métodos. A amostra contou com 24 ratos machos adultos, da linhagem Wistar-Tecpar, divididos aleatoriamente em três grupos com oito animais cada. O Grupo Controle Negativo (G.C), não foi exposto às substâncias. O Grupo Controle Positivo (G.F) foi exposto ao formaldeído a 1.33 ppm e o Grupo Experimental (G.CP) exposto à substância Complucad®. Os animais foram mantidos sob condições normais de temperatura, com fotoperíodo de 12h claro/escuro, alimentados com ração balanceada para roedores e água ad libitum, sendo expostos diariamente (8 horas/dia) às respectivas substâncias. Após o período de exposição os mesmos foram anestesiados para coleta sanguínea, seguida de eutanásia para coleta dos tecidos. Resultados: O teste ANOVA seguido de TUKEY realizado com nível de significância de 5% demonstrou para a enzima alanina diferença (p=0,04) entre o G.F quando comparado ao controle. A enzima aspartato apresentou (p=0,20). A avaliação histológica dos órgãos demonstrou alteração significativa para o tecido hepático sendo (p=0,0001), tecido pulmonar (p=0,0085) e tecido renal (p=0,00), mediante o teste estatístico com Qui-Quadrado. Através da aplicação do teste Kruskall Wallis 5% para as variáveis dos tecidos, observou-se infiltração de células e perda da arquitetura (p<0,03), dilatação alveolar (p=0,01), tumefação celular cortical, congestão vascular cortical e dilatação tubular (p<0,01). Conclusão: Houve grau de toxicidade aos referidos xenobióticos, sendo em maior intensidade no grupo exposto ao formaldeídeo a 10%.

Objectives: Verify the toxicity in Wistar rats exposed to formaldehyde solutions at 10% and Complucad®, analyzing the alaninaaminotransferase/aspartatoaminotransferase hepatic enzymes and the lung, kidney and liver tissues, after a sharp period of exposition. Methods: The 24 Wistar-Tecpar adult male rats, divided randomly into three groups, with eight animals each group. The Negative Control Group (G.C) was not exposed to the substances; the Positive Control Group (G.F) was exposed to formaldehyde at 1.33 ppm, and the Experimental Group (G. CP) was exposed to Complucad®. The animals were kept under room temperature, in a 12 hour light/dark photo period, fed with a balanced diet for rodents and ad libitum water, being daily exposed (8 hours a day) to the respective substances. After a period of exposition, they were anesthetized for blood collection, then the euthanasia for tissue collection. Results: the ANOVA followed by TUKEY performed with a significance level of 5% showed for enzyme alanine (p=0,04) between the G.F compared with the control group. The enzyme aspartate, showed (p=0,20). The organs histological evaluation showed a significant alteration for the liver tissue, being (p=0,0001), lung tissue (p=0,085), and kidney tissue (p=0.00), before treatment with the Qui-Quadrado Test. Applying the test Kruskall Wallis 5% for the variables of the tissues, observed, cell infiltration and loss of architecture (p=0,03), dilated alveolar (p=0,01), cortical cell swelling, cortical vascular congestion and dilatation tubular (p<0,01). Conclusion: There was a degree of toxicity to the referred xenobiotic, being more intense on the group exposed to formaldehyde at 10%.

Study on physical and some serum parameters of inhabitants living in agricultural Terai region, Nepal.

Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyltranspeptidase (gamma-GTP), lactate dehydrogenase (LDH), urea nitrogen (BUN), uric acid (UA) and creatinine (CRE) levels were examined in 183 people (98 males and 85 females aged 10 to 68 years) living in Terai region in Nepal. The mean values of serum components examined did not differ by sex in the age group of 10-14 years. The mean values of serum AST, ALT and gamma-GTP levels differed significantly between the sexes (P<0.01). The all physical measurements and serum parameters observed correlated well in males, but a few of them correlated in females. The AST-ALT, AST-gamma-GTP, ALT-CRE and BUN-CRE correlated well in both sexes. The UA correlated with ALT and gamma-GTP, CRE with gamma-GTP, LDH and UA in males, while only AST-LDH and gamma-GTP-BUN correlated in females. The levels of most of the serum components examined in this study were within normal ranges for Japanese and others. However, it seemed to be necessary to improve their living conditions as these serum components are related to hepatic or renal function and the infectious diseases.